Paper
Dynamic Modeling, Simulation and in silico Optimization of Fructooligosaccharides by Immobilized Fructosyltransferase in Continuous Basket Reactor
- Authors:
- Francisco Maugeri; Mónica Alvarado-Huallanco
- Abstract
- Modeling and simulation studies represent powerful tools, saving time and resources for the development of bioprocesses. Thus, studies of modeling, simulation and production of fructooligosaccharides, from sucrose, with fructosyl transferase from Rhodotorula sp. have been performed lately. In this paper, dynamic modeling of the synthesis process in continuous basket bioreactor was carried out, from mass balances and reaction kinetics, equally contemplating the resistance to mass transfer phenomena. The computational tools used were the Simulink programs of the MatLab 6.0 software, MathWork, Inc, USA. The model obtained, together with the simulation process, were used for the in silico optimization of the continuous production of fructooligosaccharides by fructosyl transferase immobilized in an inorganic support. A factorial design methodology was used, performing a central composite rotational design (CCRD). The optimum conditions obtained were: 4 Ui/mL of biocatalytic activity, stirring speed of 35 rpm, sucrose feed concentration of 550 g/L and residence time of 29 h. The other process conditions such as pH, temperature and substrate concentration were similar to those obtained in previous studies. Thus, in optimal conditions, the yield of FOS was around 31.2% and productivity around 5.4 gLh, sucrose feed concentration of 50%, 50C of temperature and pH 4.5. The results showed that, for the continuous reactor, the FOS yield was about 31%, lower than in batch process; however the main FOS fraction was GF4, around 30.4% of the total, a different result from those obtained by other types of reactors. Finally, biocatalytic activity and mass transfer coefficient had significant influence on the course of the reaction and on the FOS yield.
- Keywords
- Fructosyltransferase; Rhodotorula sp.; Fructooligosaccharides; Immobilized Enzyme; Continuous Basket Reactor; Mathematical Modeling; Simulation and Process Optimization
- StartPage
- 27
- EndPage
- 33
- Doi