Paper
Detection of Human Papilloma Virus DNA from Dry Paper Cervical Smear- a Hospital Based Study
- Authors:
- Usha Sarma; Jagadish Mahanta; Biswajyoti Borkakoty; Kunja L. Talukdar
- Abstract
- Background: Persistent infection of uterine cervix by human papilloma virus (HPV) among which high risk HPV (16, 18, 31, 33, 35, 52, and 58 collectively) are associated with cervical carcinogenesis. Cervical cancer precedes a precursor stage termed as cervical dysplasia or intraepithelial neoplasia which takes long years for development of invasive cancer. Aim: To studythe prevalence of HPV infection in uterine cervix and to correlate the HPV status of the cervical scrapping with the routine method of cervical cytology by Papanicolaou stain. Methodology: The studyincluded 226 no ofcervical scrapping from married non pregnant women attending gynecology OPD for both Pap smear and PCR testing to detect HPV DNA. The Nested PCR using primers for L1 consensus gene with My09/My11 & GP6+/GP5+ used for screening HPV followed by multiplex PCR which was carried out to detect HPV 16 & HPV18 by respective primers. Result: One hundred and ninety nine Papsmears were reported as NILM and 27 smears showed presence of SIL and invasive cervical carcinoma. PCR results detected9.7% of the patientsto be HPV positive out of which 5.3% cases were HR HPV 16 positive, 2.6 % cases were HPV 18 positive and 1.8% cases were HPV positive other than HPV 16 & 18.The sensitivity and specificity of the study were0.8182 (95%CI 0.5974-0.9481) and 0.9559 (95% CI 0.9178-0.9796) with positive predictive value being 0.6667(95% CI 0.4600-0.8349).
- Keywords
- Human Papiloma Virus; Pap Smear; Nested PCR; Dysplasia; SIL
- StartPage
- 83
- EndPage
- 88
- Doi
- 10.5963/LSMR0302006