Paper
In Vivo Targeted Ionic Calcium Stores by Naturally Occurring Oxalates
- Authors:
- Benjamin J. Scherlag; Manuel Menes; Sunny S. Po; Abraham A. Embi
- Abstract
- Targeted in vivo calcium chelation is proposed as an experimental treatment in the interruption of the infectivity of the life cycle of parasites. Calcium chelators in various millimolar concentrations were injected in vivo in dogs. The Intrinsic Cardiac Nervous System was identified and infiltrated. Histological studies showed that calcium chelators had infiltrated the sympathetic atrial plexus and the underlying atrial myocardium. This allowed visualizing the calcium stores of atrial myocardial and neural tissue. Histological samples processed and stained via the calcium specific von Kossa technique converting the ionic calcium to the carbonate (stained black). Those images and their interpretation are discussed in this manuscript. Previous research showed that Ca++ response is essential in mammalian cell invasion of parasites. In Chagas’ disease pre-treatment with calcium chelators of Trypanosome cruzi (T. cruzi) trypomastigotes has decreased the trypomastigotes’ association to cells by 40 and 63%. Methodology for oxalates induced calcium chelators delivered to parasite nests is presented. A search of the medical literature, failed to show any in vivo microinjection of chelators as described in this report.
- Keywords
- Calcium Chelation; Chagas Disease; Von Kossa; Targeted Nanoparticles; Ionic Calcium; Intracellular Chelation; Reentrant Arrhythmias; Parasitic Therapy; Cancer Biosignals
- StartPage
- 42
- EndPage
- 47
- Doi