Volume 4 Issue 6

Authors: Suchada Phunturug; Pimmada Kesrak

Abstract: Fluoride-releasing materials are commonly used to restore cervical lesions and in reconstructive periodontal surgery. The attachment and proliferation of various materials on human periodontal ligament fibroblast (HPDL) cells were evaluated in this study. Four fluoride-releasing restorative materials were tested: GIC (F9), RMGIC (F2), Giomer (B), and resin composite (G); while resin composite (P) was used as a negative control. The specimens were prepared according to the standard protocol, and then primary cultures of HPDL cells were seeded on specimens and control glass cover slips. The attached cells were counted at 1, 3, 24, and 72 h after cell seeding. Cell proliferation was determined using MTT assay at 1, 3, 5, and 7 days after cell seeding. The cell morphology was determined by SEM. Cell attachment increased as time elapsed for all materials. After initial attachment, Phad the best cell attachment profile when compared to the other groups. G had the greatest amount of cell attachment at the end of cell culture time, while F9 showed the least. The cell proliferation profile of G was the highest, while F9 and F2 were the least (p < 0.05) among all groups. SEM evaluation determined that HPDL cells on the materials were round or oval at the initial time of cell culture. As time elapsed, HPDL cells presented a variety of cell morphologies in the development of cytoplasmic processes.

Keywords: Attachment Potential; Fluoride-Releasing Restorative Materials; Periodontal Fibroblast; Proliferation Potential