Paper
Application of FT-IR Spectroscopy Coupled with Chemometrics for Authentication of Taxus chinensis (Pilger) Rehd
- Authors:
- Changshun Wu; Jiani Shou; Peng Yu; Cungui Cheng
- Abstract
- Horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FT-IR) is to measure the HATR-FT-IR spectra of leaves, epidermis and xylem of Taxus chinensis, Cryptomeria fortunei, Taxodium distichum, and Metasequoia glyptostroboides. Cluster analysis was used to compare the characteristic absorption peaks in different organs of Taxus chinensis, Cryptomeria fortunei, Taxodium distichum, and Metasequoia glyptostroboides, and the result showed that the classification based on the analysis of the plant epidermis was consistent with traditional plant taxonomy result, but based on leaves and xylem was inconsistent with the traditional plant taxonomy results. Then, six feature variants were extracted using discrete wavelet transformation (DWT) analysis. The extracted feature variants were then inputted into the support vector machine (SVM) for classification of Taxus chinensis and other plants. Among the kernel functions the SVM used, the Poly and RBF kernels had the highest accuracy rates. The accuracy of the Poly kernel in Taxus chinensis was 98.33%. The results indicated that HATR - FT-IR method was combined with chemometrics to apply in the Identify research among Taxus chinensis, Cryptomeria fortunei, Taxodium distichum, and Metasequoia glyptostroboides.
- Keywords
- Horizontal Attenuated Total Reflection Fourier Transform Infrared Spectroscopy; DWT; SVM; Taxus Chinensis; Identification
- StartPage
- 61
- EndPage
- 69
- Doi