Paper
Decellularization of Xenogenic Bone Grafts for Potential Use as Tissue Engineering Scaffolds
- Authors:
- To Minh Quan; Doan Nguyen Vu; Nguyen Thi Ngoc My; Tran Le Bao Ha
- Abstract
- Xenogenic bone grafts have been studied as an alternative to autogenic and allogenic bone grafts in order to satisfy the high demand for orthopedic reconstruction. The aim of the present study was to develop an effective method for xenogenic bone decellularization and cells reseeding forwards bone regeneration applications. Decellularization of porcine cancellous bone blocks with either acetone or chloroform/methanol results in completely acellular bone scaffolds. Umbilical cord blood-derived cells were seeded on the acellular cancellous bone by centrifugal force 2000 rpm in 1 minute and repeated 4 times. After 7 days of in vitro culture, the cells adherence and proliferation within the scaffolds were confirmed by MTT and SEM. In vivo transplantation of acellular bone scaffolds also demonstrated that they did not cause immune response. The achieved results demonstrated that the acellular bone scaffolds are capable of supporting cell adherence and proliferation. Furthermore, in vivo study shows the potential use of acellular bone scaffolds as bone grafts in orthopedic surgery.
- Keywords
- Xenogenic Bone Graft; Decellularization; Scaffold; Bone Regeneration; Cancellous Bone; Umbilical Cord Blood-Derived Cells; Cell-Seeding
- StartPage
- 38
- EndPage
- 45
- Doi
- 10.5963/LSMR0404001