Genomic Template Stability Variation in Zebrafish Exposed to Pharmacological Agents

Vincenzo Stingo; Carmela Peluso; Ippolita Veronica Valentino; Lucia Rocco
Genotoxic agents, including drugs, compromise the integrity of genetic material and influence DNA expression, both directly and indirectly. Notwithstanding the fact that the large selection of pharmacological molecules has improved the health of individual humans and/or animals, they have also caused a notable damage to the environment due to their high reactivity. In the present study we evaluated genetic effects of nine pharmacological substances in 170 individuals of Danio rerio using RAPD-PCR analysis. The amplification products from the individuals exposed to the same concentrations of the environmental pollutants showed significant changes in their electrophoretic pattern with respect to the negative controls. The genotoxic activity of these drugs was also validated at the molecular level by means of mathematical and statistical approaches. The aim of this study was to confirm genotoxicity data previously obtained by the Comet test and Diffusion assay using the RAPD technique, comparing changes in RAPD profiles and evaluating the loss of genomic stability (GTS, %). These results show that all drugs have toxic effects on genomic template stability at the concentrations used.
Pharmacological Agents; Genotoxicity; RAPD-PCR; GTS; Zebrafish
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