Paper
Analysis of Selenium in Medical, Food and Liquid Healthcare Samples by Means of Total Reflection X-Ray Fluorescence (TXRF) Spectrometry
- Authors:
- Hagen Stosnach
- Abstract
- In this paper the possibilities and restrictions of TXRF spectroscopy for the analysis of selenium in different sample types are evaluated. Liquid medical samples like urine were accurately analysed for selenium contents in the range of 4.2 to 91 µg/l via quantification with internal standardisation. Additionally, successful quantification of selenium in blood serum, whole blood, seminal plasma, hair samples, placenta and laboratory mice tissue is reported, covering a concentration range from 36 µg/l (human seminal plasma) to 2735 mg/kg (laboratory mice tissue). Solid food samples were analysed after slurry preparation and microwave aided acid digestion. The analysis of various certified reference materials proved that an accurate analysis of selenium by means of TXRF is possible with both approaches. This was confirmed by TXRF and ICP-MS analysis of different animal tissue and feed samples. Similar results were obtained for the analysis of selenium in liquid healthcare products by means of TXRF and ICP-OES analysis. In summary it was found that even though detection limits are above ICP-MS and measurement times are longer, compared to established techniques, TXRF is a suitable supplementary tool for selenium analysis. Major advantages of this method are the extremely easy sample preparation and quantification as well as the low sample amounts (few µl) needed.
- Keywords
- Selenium; Medical Sample; Food Samples; Health Care Samples
- StartPage
- 121
- EndPage
- 126
- Doi