Volume 1 Issue 2
Authors: Feng Qin
Abstract: Traditional Chinese medicine prescriptions (TCMP) is playing an indispensable role in the prevention and treatment of diseases in China and East Asia area for many years. Biomedical analysis is crucial to elucidate the mechanism of TCMPs. At present, a large number of papers have been published in the pharmaceutical and biomedical analysis of TCMPs, stemming mainly from modern separation, characterization techniques, sample extraction methods and so on. Liquid chromatography, gas chromatography, capillary electrophoresis and enzyme immunoassay are commonly used to study the TCMPs. In the review, the development and application of qualitative and quantitative analysis in biomedical analysis of TCMPs are discussed. Then, we also expound the applications of biomedical analysis in TCMPs. Finally, we discussed some future research requirements for the domain.
Keywords: Traditional Chinese Medicine; Biomedical Analysis; Pharmacokinetics; Qualitative; Quantitative
Authors: Jia Tang; Xiao Ru Li; Jiao Han
Abstract: The aim of this paper is to analysis the volatile constituents in rhizome zingibers (RZ), zingiber officinale roscoe (ZOR) and ginger pee (GP).The similarities and differences of volatile components in RZ, ZOR and GP were investigated by GC-MS combined with a chemometric method named heuristic evolving latent projections (HELP). And temperature-programmed retention indices (PTRIs) were used together with mass spectra for identification of the essential oil components. In total, 85, 81 and 80 volatile components in volatile oil of RZ, ZOR and GP were separately determined qualitatively，accounting for 81.43%, 86.38% and 84.79% of total contents of volatile oil in RZ, ZOR and GP respectively. The results indicate that there were total 58 common compounds existing in RZ and ZOR, 63 common components between RZ and GP, 60 common components between ZOR and GP, and 52 common components existing among the three systems. The results obtained may be helpful to the further study of pharmacological activity for their potential utilization as therapeutical agents.
Keywords: Volatile Constituents; Heuristic Evolving Latent Projections (HELP); GC-MS; Temperature-Programmed Retention Indices (PTRIs); Identification
Authors: Chien Chun Wang; Liliana Fernande
Abstract: In aqueous solutions, sildenafil presents a very low fluorescence emission and a limited linear range. In presence of a cationic surfactant hexadecyltrim ethylammonium bromide (HTAB, method A) and anionic surfactant sodium dodecyl sulfate (SDS, method B) a great fluorescence enhancement was observed and the linearity range was enlarged. These surfactants-drug interactions lead to the development of two sensitive methods for sildenafil spectrofluorimetric determination. Nature of interactions between sildenafil and surfactants were studied and different parameters which influence these associations were discussed. Sildenafil was quantitatively determined at an emission wavelength of 435 nm and 415 nm by method A and B respectively with detection limit of 0.0012 µg mL-1 and 0.0016 µg mL-1. The methods have been successfully applied to the analysis of bulk drug, tablets and herbal medicines.
Keywords: Pharmaceutical Analysis; Fluorimetric Analysis; Surfactants; Sildenafil